e-journal

Involvement of nitric oxide and auxin in signal transduction of copper-induced morphological responses in Arabidopsis seedlings

---

Background and Aims Plants are able to adapt to the environment dynamically through regulation of their
growth and development. Excess copper (Cu2+ ), a toxic heavy metal, induces morphological alterations in
plant organs; however, the underlying mechanisms are still unclear. With this in mind, the multiple signalling functions of nitric oxide (NO) in plant cells and its possible regulatory role and relationship with auxin were examined during Cu2+-induced morphological responses.
Methods Endogenous auxin distribution was determined by microscopic observation of X-Gluc-stained
DR5::GUS arabidopsis, and the levels of NO, superoxide and peroxynitrite were detected by fluorescence
microscopy. As well as wild-type, NO-overproducer (nox1) and -deficient (nia1nia2 and nia1nia2noa1-2) arabidopsis plants were used.
Key Results Cu2+at a concentration of 50 mM resulted in a large reduction in cotyledon area and hypocotyl and primary root lengths, accompanied by an increase in auxin levels. In cotyledons, a low Cu2+
concentration promoted NO accumulation, which was arrested by nitric oxide synthase or nitrate reductase inhibitors. The 5-mM Cu2+
induced NO synthesis was not detectable in nia1nia2 or nia1nia2noa1-2 plants. In roots, Cu2+
caused a decrease of the NO level which was not associated with superoxide and peroxynitrite formation. Inhibition of auxin transport resulted in an increase in NO levels, while exogenous application of an NO donor reduced DR5::GUS expression. The elongation processes of nox1 were not sensitive to Cu2+
, but NO-deficient plants showed diverse growth responses.
Conclusions In plant organs, Cu2+ excess results in severe morphological responses during which the endogenous hormonal balance and signal transduction are affected. Auxin and NO negatively regulate each other’s level and NO intensifies the metal-induced cotyledon expansion, but mitigates elongation processes under Cu2+ exposure.
Key words: Arabidopsis thaliana, auxin, copper, morphological responses, nitric oxide.

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

-

Penerbit

: Oxford University Press., 2011

Deskripsi Fisik

Annals of Botany 108: 449–457, 2011

Bahasa

English

ISBN/ISSN

-

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

PERTANIAN.

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Yuli/Agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar