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Functional characterization of terpene synthases and chemotypic variation in three lavender species of section Stoechas

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Lavandula pedunculata (Mill.) Cav. subsp. lusitanica, Lavandula stoechas L. subsp. stoechas and Lavandula viridis l’Hér. are three lavender taxa that belong to the botanical section Stoechas and are widely used as aromatherapy, culinary herb or folk medicine in many Mediterranean regions. The analysis of their bioactive volatile constituents revealed the presence of 124 substances, the most abundant being the bicyclic monoterpenes fenchone, camphor and 1,8-cineole that give these three species their respective chemotypes. Most noteworthy was fenchone which, with its reduced form fenchol, made 48% of the total volatile constituents of L. pedunculata while present at 2.9% in L. stoechas and undetectable in L. viridis. In order to provide a molecular explanation to the differences in volatile compounds of these three species, two monoterpene synthases (monoTPS) and one sesquiterpene synthase (sesquiTPS) were cloned in L. pedunculata and functionally characterized as fenchol synthase (LpFENS), α-pinene synthase (LpPINS) and germacrene A synthase (LpGEAS). The two other lavender species contained a single orthologous gene for each of these three classes of TPS with similar enzyme product specificities. Expression profiles of FENS and PINS genes matched the
accumulation profile of the enzyme products unlike GEAS. This study provides one of the rare documented cases of chemotype modification during plant speciation via changes in the level of plant TPS gene expression, and
not functionality.

Abbreviations – BSA, bovine serum albumin; DTT, dithiothreitol; FENS, fenchol synthase; FPP, farnesyl pyrophosphate;GC-MS, gas chromatography-mass spectrometry; GEAS, germacrene A synthase; GGPP, geranyl geranylpyrophosphate; GPP, geranyl pyrophosphate; monoTPS, monoterpene synthases; PINS, pinene synthase; RI, retention indices; RT-PCR, reverse transcription-polymerase chain reaction; sesquiTPS, sesquiterpene synthase; SfCinS, Salvia fruticosa 1,8-cineole synthase; SpSabS1, Salvia pomifera sabinene synthase; TIC, total ion current; TPS, terpene synthase.

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Informasi Detail

Judul Seri

Physiologia Plantarum

No. Panggil

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Penerbit

Scandinavian : Scandinavian Plant Physiology Society., 2015

Deskripsi Fisik

Physiologia Plantarum 153: 43–57. 2015

Bahasa

English

ISBN/ISSN

0031-9317

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

153: 43–57. 2015

Subjek

FISIOLOGI TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

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