e-journal

Molecular tagging of Asiatic soybean rust resistance in exotic genotype EC 241780 reveals complementation of two genes

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Asian soybean rust (ASR) caused by Phakopsora pachyrhizi severely reduces seed yield in soybean. Molecular tagging of ASR resistance can help in the process of resistance breeding. In this study, an F2 population
of cross (susceptible cultivar ‘NRC 7’ 9 resistant exotic genotype EC 241780) was used for bulked segregant analysis (BSA) with 25 SSR (simple sequence repeat) primers linked with six Rpp genes. Among them, five polymorphic SSR markers, viz., Sct 187, SSR 1859, Satt 191 (Rpp1b like loci) and Satt 215, Sat_361 (Rpp2 loci) distinguished the ASR resistant and susceptible bulks and individuals. In combined marker analysis, the markers Satt 191 (Rpp1b like loci) and Satt 215 (Rpp2 loci) were linked with ASR severity score and were also confirmed in individual 110 F2 segregants. Hence, these markers could be utilized in the marker assisted rust resistance breeding of Rpp1b like and Rpp2 genes. In silico candidate gene analysis for hypersensitive response revealed that Satt 191 linked region was rich in genes encoding apoptotic ATPase having leucine-rich repeat (LRR) domain.

Key words: soybean — Asian soybean rust — simple sequence repeat markers — bulked segregant analysis — Rpp genes

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Ketersediaan

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Informasi Detail

Judul Seri

Plant Breeding

No. Panggil

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Penerbit

New York : Blackwell Verlag GmbH., 2015

Deskripsi Fisik

Plant Breeding, 134, 70–77 (2015)

Bahasa

English

ISBN/ISSN

doi:10.1111/pbr.1224

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

134, 70–77 (2015)

Subjek

PEMULIAAN TANAMAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

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