e-journal

Epigallocatechin gallate inhibits the HIV reverse transcription step

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Background: Epigallocatechin gallate (EGCG), the most abundant catechin in green tea, has been reported to
inhibit HIV-1 replication prior to its integration into host DNA via various proposed mechanisms; however, the specific
main target(s) of EGCG remain unclear. In this study,we investigated a number of these proposed detailed mechanism(s) using a cell-based model.Methods: Multinuclear activation of galactosidase indicator assays were used for all experiments, including examination of the time of addition and the synergisms with a nucleoside reverse transcriptase inhibitor,3′-azido-3′-deoxythymidine (AZT). Results: The experiments revealed that EGCG suppressed
both HIV-1IIIB and HIV-2EHO infection in HeLa-CD4-LTR-β-gal cells, with relatively low 50% effective concentrations
of 1.6 and 2.0 μM, respectively. The inhibitory profile of EGCG generated using a time-of-addition assay was
identical to that of a non-nucleoside reverse transcriptase inhibitor (NNRTI), MKC-442. Furthermore, synergistic
inhibition was observed in EGCG with AZT. onclusions: Based on our findings, EGCG appears to act mainly as an allosteric reverse transcriptase inhibitor with mechanisms different from those of currently
approved NNRTIs that directly interact with the NNRTI binding pocket. Thus, EGCG is a good candidate for use
as an additional or supportive anti-HIV agent derived from natural plants.

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Informasi Detail

Judul Seri

Antiviral Chemistry & Chemotherapy

No. Panggil

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Penerbit

: International Medical Press., 2011

Deskripsi Fisik

Antiviral Chemistry & Chemotherapy 2011; 21:239–243

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KESEHATAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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