e-journal
Validation of an Animal Isolation Imaging Chamber for Use in Animal Biosafety Level-3 Containment
Live imaging of animals infected with pathogenic microbes poses a contamination risk to equipment, personnel, and other animals. A Caliper animal isolation chamber designed for the IVISĀ® Spectrum imaging system was tested as a containment device for mice infected with microbes assigned to animal biosafety level-3 (ABSL-3). A testing protocol was developed by adapting two published standards to test other equipment in high-containment environments. The protocol included quantitative leak-testing of the high efficiency particulate air (HEPA) filters, soap bubble testing of the animal isolation chamber, and pressure decay testing of the complete containment system. HEPA filters were >99.999% efficient (
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