e-journal

The CRC orthologue from Pisum sativum shows conserved functions in carpel morphogenesis and vascular development

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† Background and Aims CRABS CLAW(CRC) is a member of theYABBY family of transcription factors involved
in carpel morphogenesis, floral determinacy and nectary specification in arabidopsis. CRC orthologues have been functionally characterized across angiosperms, revealing additional roles in leaf vascular development and carpel identity specification in Poaceae. These studies support an ancestral role of CRCorthologues in carpel development, while roles in vascular development and nectary specification appear to be derived. This study aimed to expand research on CRC functional conservation to the legume family in order to better understand the evolutionary history of CRC orthologues in angiosperms.

† Methods CRC orthologues from Pisum sativum and Medicago truncatulawere identified. RNA in situ hybridization experiments determined the corresponding expression patterns throughout flower development. The phenotypic effects of reduced CRC activity were investigated in P. sativum using virus-induced gene silencing.

† Key Results CRC orthologues from P. sativum and M. truncatula showed similar expression patterns, mainly
restricted to carpels and nectaries. However, these expression patterns differed from those of other core eudicots, most importantly in a lack of abaxial expression in the carpel and in atypical expression associated with the medial vein of the ovary. CRC downregulation in pea caused defects in carpel fusion and style/stigma development, both typically associated with CRC function in eudicots, but also affected vascular development in the carpel.

†Conclusions The data support the conserved roles of CRC orthologues in carpel fusion, style/stigma development and nectary development. In addition, an intriguing newaspect of CRC function in legumeswas the unexpected role in vascular development, which could be shared by other species from widely diverged clades within the angiosperms, suggesting that this role could be ancestral rather than derived, as so far generally accepted.

Key words: Flower development, CRABS CLAW, CRC orthologues, carpel evolution, gynoecium, nectary, carpel
vasculature, floral determinacy, Pisum sativum, Medicago truncatula, YABBY transcription factors.

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Informasi Detail

Judul Seri

Annals of Botany

No. Panggil

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Penerbit

Oxford : Oxford University Press on behalf of the Annals of Botany Company., 2014

Deskripsi Fisik

Annals of Botany 114: 1535–1544, 2014

Bahasa

English

ISBN/ISSN

doi:10.1093/aob/mcu1

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

114:2014

Subjek

BIOLOGI
BOTANI

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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