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Antimicrobial-resistant genes associated with Salmonella spp. isolated from human, poultry, and seafood sources

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Antimicrobial-resistant salmonellosis is a significant public health concern globally.
A study was conducted to screen for Salmonella species from a total of 120
samples, of which 50 were retail meat samples purchased from five randomly
selected sales outlets in the city of Mangalore, India. Twenty poultry fecal materials
freshly voided before slaughter were obtained with sterile spatula and
placed in sterile sealable polythene envelopes, and 20 clams were purchased
from the estuaries of Nethravathi and Kankarnady market. In addition, 30 clinical
isolates from Nigeria suspected to be Salmonella by only cultural characterization
were also included in the study. In all, 30 samples—6 poultry, 8
seafood, and 16 Salmonella isolates from clinical samples—were confirmed
positive by PCR and used in this study. The disk-diffusion test was performed
to determine the zone of inhibition, and detection of resistance genes was tested
by PCR targeting various antimicrobial genes. Resistance to tetracycline (TET),
cotrimoxazole, nalidixic acid, nitrofurantion, and piperacillin/tazobactin was
found in 66.7%, 60%, 53.3%, 50% and 50% of the isolates, respectively. About
60–100% of MDR isolates possessed antibiotic-resistant genes, of the tetracyclines
resistant isolates, 20 (100%) 6 (30%), 7 (35%), and 10 (50%) carried
tetA, tetB, tetC, and tetG genes, respectively. Of 18 cotrimoxazole-resistant
strains, 18 (100%), 14 (77.7%), and 4 (22.2%) had sul1, sul2, and sul3 genes,
respectively. Of the 14 multidrug-resistant isolates tested, 8 (61%) and 9 (69%)
were positive for cmlA and cmlB genes, respectively, 10 (1.4%) tested positive
for aph(3)11a genes, 8 (57%) tested positive for aac(3)lla, while none was positive
for the aac6 gene. The results show the presence of antibiotic-resistant Salmonella
spp. in food samples from India and in human samples from Nigeria.

Keywords
Antimicrobial resistance, India, Nigeria, resistant genes, Salmonella

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Informasi Detail

Judul Seri

Food Science & Nutrition

No. Panggil

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Penerbit

New York : Wiley Periodicals, Inc.., 2014

Deskripsi Fisik

Food Science & Nutrition 2014; 2(4): 436– 442

Bahasa

English

ISBN/ISSN

doi: 10.1002/fsn3.11

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

2014; 2(4)

Subjek

GIZI DAN PANGAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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