e-journal

Genetic analysis of ORF5 porcine reproductive and respiratory syndrome virus isolated in Vietnam

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Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically
important swine pathogens because it is highly infectious and causes economic losses due to decreased
pig productivity. In this study, the 603 bp complete major envelope protein encoding gene (ORF5) of 32
field PRRSV isolates from Vietnam collected during 2008–2012 were sequenced and analyzed.Multiple
nucleotide (nt) and deduced amino acid (aa) alignments of ORF5 were performed on the 32 isolates: the
representative strains (European and North American genotypes), Chinese strains available in
GenBank and vaccine strains licensed for use in Vietnam. The results showed 94.8–100.0% nt identity
and 94.0–100% aa similarity among the 32 isolates. These isolates shared similarities with the prototype
of the North American PRRSV strain (VR‐2332; nt 87.8–89.3%, aa 87.5–90.0%), and Lelystat virus, the
prototype of the European PRRSV strain (LV; nt 61.1–61.9%, aa 55.1‐57.0%). There was greater
similarity with QN07 (nt 96.5‐98.5%, aa 96.0‐99.0%) from the 2007 PRRS outbreak in QuangNam
Province, CH‐1a (nt 93.2–95.1%, 91.5–93.5%) isolated in China in 1995 and JXA1 (nt 96.5–98.6%, aa
95.0–98.0%), the highly pathogenic strain from China isolated in 2006. The Vietnamese isolates were
more similar to JXA1‐R (nt 96.5–98.6%, aa 95.0–98.0%), the strain used in Chinese vaccines, than to
Ingelvac MLV/BSL‐PS (nt 87.2–89.0%, aa 86.0–89.0%). Phylogenetic analysis showed that the 32
isolates were of the North American genotype and classified into sub‐lineage 8.7. This sub‐lineage
contains highly pathogenic Chinese PRRSV strains. This study documents genetic variation in
circulating PRRSV strains and could assist more effective use of PRRS vaccines in Vietnam.

Key words genetic variation of ORF5, North American genotype, phylogenic analysis, porcine reproductive and
respiratory syndrome. Author: This phrase has been reworded for clarity. Has the meaning been retained?.

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Informasi Detail

Judul Seri

Microbiol Immunol

No. Panggil

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Penerbit

: The Societies and Wiley Publishing Asia., 2013

Deskripsi Fisik

Microbiol Immunol 2013; 57: 518–526 doi: 10.1111/1348-0421.12067

Bahasa

English

ISBN/ISSN

doi: 10.1111/1348-04

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

2013; 57

Subjek

KESEHATAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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