e-journal

Effect of 1‐methyl‐D‐tryptophan and adoptive transfer of dendritic cells on polymicrobial sepsis induced by cecal content injection

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A mouse model of polymicrobial sepsis induced by cecal content injection (CCI) was developed with the
aim of gaining a better understanding of the mechanism of sepsis. This model has a similar survival
pattern to the conventional model with the added benefits of ability to vary the severity of sepsis and
greater consistency. Administration of 1‐methyl‐D‐tryptophan (1‐MT) to inhibit indoleamine 2,3‐
dioxygenase (IDO) in mice with CCI‐induced sepsis increased the survival rate and tended to upregulate
IL‐10/IL‐12 serum concentrations. The effectiveness of 1‐MT was confirmed by increases in
IL‐10 over IL‐12 in bone marrow‐derived dendritic cells (BMDCs) treated with LPS and 1‐MT and a
superior survival rate 24 hr after injection of these double treated BMDCs in the CCI‐induced sepsis
model. Therefore, CCI is both a useful and reliable technique for investigating polymicrobial sepsis.
The present findings using this newly developed model suggest that inhibition of IDO alleviates the
severity of polymicrobial sepsis and modulates the immune response even in cases of severe systemic
septic inflammation.

Key words clinical immunology, dendritic cells, innate immunity.

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Ketersediaan

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Informasi Detail

Judul Seri

Microbiol Immunol

No. Panggil

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Penerbit

: The Societies and Wiley Publishing Asia., 2013

Deskripsi Fisik

Microbiol Immunol 2013; 57: 633–639 12081

Bahasa

English

ISBN/ISSN

doi: 10.1111/1348-04

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KESEHATAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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