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The origin of reactive oxygen during interaction of Pinus sylvestris root and Heterobasidion annosum s.l. – the linkage with the iron

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Reactive oxygen species (ROS) can be promoted differently, although iron is regarded as a condicio sine qua of their generation. We assessed how enzymes involved in ROS production and iron accumulation impact the interactions between Pinus sylvestris root and pathogens with di fferent host preferences (Heterobasidion annosum sensu stricto, H. parviporum and H. abietinum). Studies with inhibitors of ROS generation showed that diphenylene iodonium (DPI) and potassium cyanide (KCN) decreased the mortality of P. sylvestris root cells when exposed to H. parviporum in the elongation zone. In the meristematic zone, inhibited production of NADPH oxidase and oxalic acid decreased mortality of host root cells challenged with H. parviporum or H. abietinum. In the meristematic zone, iron immobilization with desferoxamine (DFO), inhibited percentage of dead host cells exposed to H. annosum s.s. Scots pine inoculation with high compatible H. annosum s.s., irrespectively in the root zone, led to an accumulation of superoxides (O2−) and a colocalisation of H2O2 with ferric or ferrous ions involved in production of the most harmful ROS – hydroxyl radicals at 4 h. After 48 h of mutual interaction between P. sylvestris and H. annousum s.s, a similar pattern as at 4 h was found in the root meristematic zone. We also showed that O2− accumulation in the P. sylvestris –H. annosum s.s. pathosystem was not NADPH oxidase dependent. During the interaction of P. sylvestris and H. annosum s.s., colocalisation of H2O2 with Fe2+ and
Fe3+ decreased by disturbing ROS production; this pattern was less apparent when H. parviporum or H. abietinum were identified as the invader. Our work implies that different mechanisms related to plant cell death can operate in both the root elongation and meristematic zone of P. sylvestris colonized with H. annosum s.s. pathosystem.

Keywords Iron . NADPH oxidases . Pathogen . Peroxidases . Scots pine

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Ketersediaan

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Informasi Detail

Judul Seri

Eur J Plant Pathol

No. Panggil

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Penerbit

New York : Springer., 2015

Deskripsi Fisik

Eur J Plant Pathol (2015) 143:277–290

Bahasa

English

ISBN/ISSN

DOI 10.1007/s10658-0

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

(2015) 143

Subjek

PERTANIAN.

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

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