e-journal

Structural characterization and localization analysis of the root-knot nematode Meloidogyne javanica fatty acid and retinol binding protein (Mj-FAR-1)

---

Plant-parasitic nematodes are extremely destructive pathogens with a cosmopolitan distribution and a host range that affects most crops. They are characterized by distinct parasitic lifestyles, e.g., as sedentary
or migratory endo- or ectoparasites, resulting in high losses in yield and revenue. Possessing limited lipid metabolism, they produce one or two structurally unique classes of small α-helix-rich fatty acid and retinol binding (FAR) proteins that have no counterpart in other organisms. We investigated the sequence and structural characteristics of the FAR protein of the root-knot nematode Meloidogyne javanica (Mj-FAR-1) in comparison to other studied FAR proteins. Protein sequence analyses enabled phylogenetic clustering according to trophic groups and lifestyles. Bioinformatics analysis of the FAR protein sequences revealed ten likely core amino acids representing the trophic-group clustering. Clear modifications of four of these amino acids from less reactive (nonpolar, with aliphatic R group) to more reactive (positively or negatively charged R groups, or uncharged polar R groups) might distinguish free-living from parasitic nematode species. Structural predictions of the mature Mj-FAR-1 protein and its ligand-binding pockets, suggest that adaptation toward parasitism is associated with increased reactivity of the second pocket residues, as well as those on the protein surface. Subcellular localization of Mj-FAR-1 with or without its signal peptide was determined by Agrobacterium infiltration of N-terminal mCherrytagged protein into Nicotiana benthamiana leaves. Intact Mj-FAR-1 with its signal peptide was predominantly localized along the plasma membrane surrounding plant cells while removing the signal peptide resulted in additional localization within the cell nucleus. The nuclear localization agreed with in-silico analysis of the Mj-FAR-1 sequence and sheds new light on its function in manipulating the plant response. Our study provides the first basic structural information and subcellular localization of the plant-parasitic Mj-FAR-1 protein.

Keywords: Fatty acid and retinol binding (FAR) protein . Plant-parasitic nematode . Root-knot nematode . Meloiodgyne javanica . Parasitism . Effector protein

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Eur J Plant Pathol

No. Panggil

-

Penerbit

New York : Springer., 2015

Deskripsi Fisik

Eur J Plant Pathol (2015) 143:133–149

Bahasa

English

ISBN/ISSN

DOI 10.1007/s10658-0

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

(2015) 143

Subjek

PERTANIAN.

Info Detail Spesifik

-

Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar