e-journal

Nitric oxide modulates redox-mediated defense in potato challenged with Phytophthora infestans

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In our experimental approach we investigated how post-infection nitric oxide-dependent signaling activated
in potato leaves was related to defense against avirulent (avr) and virulent (vr) races of Phytophthora
infestans. Results revealed that only in an incompatible response, early NO and superoxide (O2•-) generation led to peroxynitrite (ONOO−) formation and together with hydroen peroxide (H2O2) production synchronized
with SOD activity induced effective defense against avr pathogen. Early oxidative and nitrosative bursts
triggered an imbalance in redox homeostasis in inoculated tissue. To counteract that effect, a total antioxidative capacity, ascorbate and sulfhydryl (-SH) group compounds increased both synergistically and
markedly, confirming the precise mechanism of redox re-adjustment in avr oomycete -potato interaction. Moreover, the NO-coded message was stored and converted into an enhanced total SNO pool and particular Snitrosylation of targeted proteins. Overall, we identified 104 proteins typed for S-nitrosylation in mock- or
P. infestans-inoculated potato leaves. The Snitrosoproteome structure comprised a wide repertoire of proteins, i.e. defense- and redox-related. Finally, only in the incompatible interaction, NO-based signal was rewritten
on the rapid PR-1 gene and PR-2 protein activation and was tuned with a limitation of late blight disease
symptoms.

Keywords: Reactive nitrogen species . Reactive oxygen species .Lateblight .Biotin-switch .Redoxhomeostasis .
Biotic stress

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Ketersediaan

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Informasi Detail

Judul Seri

Eur J Plant Pathol

No. Panggil

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Penerbit

New York : Springer., 2015

Deskripsi Fisik

Eur J Plant Pathol (2015) 143:237–260

Bahasa

English

ISBN/ISSN

DOI 10.1007/s10658-0

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

(2015) 143

Subjek

PENYAKIT TUMBUHAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

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