e-journal

Activity-guided separation of Chromolaena odorata leaf extract reveals fractions with rice disease-reducing properties

---

An aqueous extract from the plant Chromolaena odorata [syn Eupatorium odoratum], Eupatorieae, Asteraceae, protected rice against sheath blight by induced resistance. As an initial step towards identification of the specific disease-reducing compounds, leaves from C. odorata were extracted with water and methanol and the extracts separated using a group separation system followed by analysis using capillary electrophoresis. The fractions from the extracts were tested in vitro and in planta using Bipolaris oryzae (cause of brown spot of rice) to test for their potential to reduce disease severity. Activity-guided separation of the C. odorata extracts indicated that compounds with activity could, at least partly, be isolated on a weakly acidic cation exchange column. Further purification yielded fractions with disease reducing effects of up to 72 % at 15 days after inoculation. Activity was found both in methanol and water extracts, indicating that the bioactive compound(s) are hydrophilic, low molecular weight compounds. The disease-reducing fractions did not display any direct antimicrobial effects, but data indicate that they protect the plants by induced resistance as evidenced from increased activity of β-1,3-glucanase.

Keywords: Antimicrobial effect . Bipolaris oryzae .Chromolaena odorata . Disease reduction . Induced resistance . Oryza sativa

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Eur J Plant Pathol

No. Panggil

-

Penerbit

New York : Springer., 2015

Deskripsi Fisik

Eur J Plant Pathol (2015) 143:331–341

Bahasa

English

ISBN/ISSN

DOI 10.1007/s10658-0

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

(2015) 143

Subjek

PENYAKIT TUMBUHAN

Info Detail Spesifik

-

Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar