e-journal

Transcriptome profiling of in vitro lines propagated from corm bud tip of elephant foot yam (Amorphophallus paeoniifolius) approves complete potyviruses elimination

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The most characterised Dasheen mosaic virus andmany other unreported putative viruses are involved in the mixed viral mosaic infection of elephant foot yam (Amorphophallus paeoniifolius). The in vitro propagation of corm bud tips for virus free plantlet production was carried out in three different culture phases consisting of
callusing, shoot regeneration and rooting. A 100 % survival ratewas recorded on hardening in sand: soil: coir pith (1:1:1) mixture. A total of 84 % of regenerated plantlets were found to be virus free on indexing of 21 in vitro lines with species specific/genus specific serological and molecular diagnostic techniques. Transcriptome sequencing was carried out for two randomly selected in vitro plants and a mosaic infected field sample. Not any of the known potyviruses were traced in the transcriptome profiles of supposed virus free plants thus confirming the complete potyviruses elimination. Disease symptoms or reoccurrence was not observed in the hardened virus-free lines of the plant.

Keywords: Corm bud . Callus . Elephant foot yam . RT-PCR . Transcriptome profiling . Virus free lines

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Informasi Detail

Judul Seri

Eur J Plant Pathol

No. Panggil

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Penerbit

New York : Springer., 2015

Deskripsi Fisik

Eur J Plant Pathol (2015) 143:363–371

Bahasa

English

ISBN/ISSN

DOI 10.1007/s10658-0

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

(2015) 143

Subjek

PENYAKIT TUMBUHAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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