e-journal

Automated Cell Transport in Optical Tweezers-Assisted Microfluidic Chambers

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Abstract—In this paper, we present a physics-aware, planning approach for automated transport of cells in an optical tweezers-assisted microfluidic chamber. The approach can be used for making a uniform distribution of cells inside the chamber to allow the study of a variety of biological processes, including cell signaling. Fluid forces inside the chamber, modeled using computational fluid dynamics, are incorporated into the widely used Langevin equation to simulate the motion of cells. The developed
simulator was used for building a map that contains probabilities of a cell successfully reaching one of the outlets of the chamber from different locations under the influence of the fluid flow. The developed planner not only generates collision-free paths that exploit the fluid flow inside the chamber but also utilizes the offline generated simulation data to decide suitable locations for releasing the cells. This ensures fast and robust cell transport, while minimizing the required laser power and operational time. The planner is based on the heuristic D* Lite algorithm that employs a specific cost function for searching over a novel state-action space representation. The effectiveness of the planning algorithm
is demonstrated using both simulation and physical experiments in a microfluidics-optical tweezers hybrid manipulation setup. Note to Practitioners—This paper presents computational tools necessary for integrating optical tweezers with microfluidic chambers. These two techniques are complementary in nature and combining them enables us to realize a method for fast and accurate placement of microparticles. The method presented in the paper is especially useful for accurately placing cells in an array for biological
experiments. Index Terms—D* lite, microfluidics, motion planning, optical tweezers, physics-based simulation, robotic manipulation, yeast.

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Ketersediaan

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Informasi Detail

Judul Seri

IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING

No. Panggil

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Penerbit

: ., 2013

Deskripsi Fisik

IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING, VOL. 10, NO. 4, OCTOBER 2013

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

VOL. 10, NO. 4

Subjek

TEKNIK

Info Detail Spesifik

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Pernyataan Tanggungjawab

yuli/agus

Versi lain/terkait

Lampiran Berkas

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