e-journal

Real-time PCR as a diagnostic tool for evaluating the resistance of Chinese cabbage cultivars to Verticillium wilt

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Verticillium wilt disease has become increasingly serious in many regions in China over the past few years. In this study, the widespread pathogen was identified as Verticillium longisporum (V. longisporum) by mt-SSU rDNA and cytochrome b sequence analysis. By using the primer pair HW1-F/HW1-R, which was designed based on the V. longisporum-specific rDNA-ITS sequence, a real-time PCR assay was developed for rapid and specific detection of the pathogen. The realtime PCR assay enabled sensitive quantification of V. longisporum in artificial and field-infected seedlings. This study provides a method for large-scale screening of Verticillium wilt-resistant resources for Chinese cabbage breeding.

Keywords: Brassica rapa subsp. pekinensis . Verticillium wilt . V. longisporum. Real-time PCR

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Informasi Detail

Judul Seri

Eur J Plant Pathol

No. Panggil

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Penerbit

New York : Springer., 2015

Deskripsi Fisik

Eur J Plant Pathol (2015) 143:549–557

Bahasa

English

ISBN/ISSN

DOI 10.1007/s10658-0

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

(2015) 143

Subjek

PENYAKIT TUMBUHAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

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