Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection

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Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection

Jenny N. Vo [et al.] - Nama Orang;

The purification of viruses from bananas is a complex and laborious process due to the large quantities of polysaccharides and secondary metabolites in the plant tissue. The banana streak viruses (BSVs) are generally
difficult to purify to a level that allows generation of high quality antisera. This study presents an improved
method of purification of Banana streak MY virus (BSMYV), which allowed us to generate highly specific chicken and rabbit antisera and to detect the capsid proteins for the first time. Finally, we demonstrate further enrichment of BSMYV by isoelectric focusing.

Keywords: Badnavirus . Musa . Immunoassay. Polyclonal antibodies . Liquid-phase-IEF

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Informasi Detail

Judul Seri: Eur J Plant Pathol
No. Panggil: -
Penerbit: New York : Springer., 2015
Deskripsi Fisik: Eur J Plant Pathol (2015) 143:619–626
Bahasa: English
ISBN/ISSN: DOI 10.1007/s10658-0
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: (2015) 143
Subjek: PENYAKIT TUMBUHAN
Info Detail Spesifik: -
Pernyataan Tanggungjawab: agus

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FULL TEXT: Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection

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