e-journal

Parentage assignment in hybrid abalones (Haliotis rufescens 3 Haliotis discus hannai) based on microsatellite DNA markers

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Parentage analysis in aquaculture determines genealogical relationships between broodstock and progeny when the parents are unknown. Thus, parentage analysis is a useful tool to establish pedigree reports in molecular-assisted selection programs. Here, we evaluated 10 heterologous microsatellite markers for parentage assignment in abalone hybrids produced from 43 abalone broodstocks of red abalone (Haliotis rufescens) and Japanese abalone (H. discus hannai). The allele frequencies, exclusion probabilities and broodstock contributions were calculated using CERVUS,
PAPA and GERUD software. The polymorphic information content (PIC) values showed that most of the microsatellite loci were highly informative (>0.7) and more than 90% of parentage assignment was possible with a minimum of 5–6 microsatellite markers. Parentage assignment for hybrid and pure-red progeny showed a better performance than pure Japanese progeny. This result could be due to the high level of allele loss in the parental genotypes. In addition, results indicated that only two sires contributed over 80% and 90% of red and hybrid progenies, respectively. This study gives a new molecular tool to support marker- assisted selection in abalone hybrids produced in Chile.

Keywords: hybrid abalone, Haliotis rufescens, Haliotis discus hannai, microsatellite markers, parentage assignment

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Ketersediaan

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Informasi Detail

Judul Seri

Aquaculture Research

No. Panggil

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Penerbit

Chile : John Wiley & Sons Ltd., 2013

Deskripsi Fisik

Aquaculture Research 2015

Bahasa

English

ISBN/ISSN

doi:10.1111/are.1216

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

2015, 46, 216–225

Subjek

PERIKANAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia/Agus

Versi lain/terkait

Lampiran Berkas

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