e-journal

Experimental infection of brown-marbled grouper, Epinephelus fuscoguttatus (Forskal), with Vibrio parahaemolyticus identifies parvalbumin beta-2 subunit I, alpha-2-macroglobulin, nattectin and immunoglobulin light chain, differentially expressed in resistant grouper

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The mechanisms through which brown-marbled grouper accomplishes resistance to infection, particularly
against Vibrios, are not yet fully understood. In this study, brown-marbled grouper fingerlings were experimentally infected with Vibrio parahaemolyticus, to identify disease resistance
grouper, and the serum proteome profiles were compared between resistant and susceptible candidates,
via two-dimensional gel electrophoresis (2-DE). The results showed that putative parvalbumin
beta-2 subunit I, alpha-2-macroglobulin, nattectin and immunoglobulin light chain proteins
were among proteins that significantly overexpressed in the resistant fish as compared to the susceptible group of fish, whereas apolipoprotein E and immunoglobulin light chain proteins were
observed to be differentially overexpressed in the susceptible fish. Further analysis by peptide
sequencing revealed that the immunoglobulin light chain proteins identified in the resistant and
susceptible groups differed in amino acid composition. Taken together, the results demonstrated
for the first time that putative parvalbumin beta-2 subunit I, alpha-2-macroglobulin, nattectin and
immunoglobulin light chain are among important proteins participating to effect disease resistance
mechanism in fish and were overexpressed to function collectively to resist V. parahaemolyticus
infection. Most of these molecules are mediators of immune response.

Keywords: 2D SDS-PAGE, fish disease, grouper, immunoglobulin light chain, infection, Vibrio parahaemolyticus.

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Ketersediaan

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Informasi Detail

Judul Seri

Journal of Fish Diseases

No. Panggil

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Penerbit

Malaysia : John Wiley & Sons Ltd., 2014

Deskripsi Fisik

Journal of Fish Diseases 2015, 38, 17–25

Bahasa

English

ISBN/ISSN

doi:10.1111/jfd.1219

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

-

Edisi

2015, 38, 17–25

Subjek

PERIKANAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia/Agus

Versi lain/terkait

Lampiran Berkas

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