e-journal

Valuing museum specimens: high-throughput DNA sequencing on historical collections of New Guinea crowned pigeons (Goura)

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Museum specimens are of particular importance for investigating systematics and the biogeography as well as
other aspects of the evolution of biodiversity. They are also a depository of specimens accumulated over recent
historical times and often the only way to study recently extinct or rare species. Unfortunately, most museum
specimens yield low-quality DNA limiting their generalized use in phylogenetic and population genetic studies.
Advances in sequencing technologies now offer opportunities to analyse such material even from very small
tissue. Here, we applied the Illumina technology (HiSeq) on three specimens of crowned pigeon (Goura spp.)
collected in New Guinea between 1879 and 1934. A shotgun strategy allowed us to quickly and reliably assemble
complete mitochondrial genomes and fragments of three single-copy gene phylogenetic markers even with low
double-stranded DNA quantity (9 to 69 ng). Phylogenetic assignments of these new sequences confirmed them as
Goura sequences. Therefore, our study confirms the power of using next-generation sequencing methods to
investigate the evolutionary history of species for which access to fresh samples is limited but museum collections
are available. These approaches are considerably increasing the value of many natural history collections for
genetic investigations, particularly from biodiversity hotspots that are presently difficult to prospect.

ADDITIONAL KEYWORDS: genome skimming – historical museum collection – mitochondrial DNA –next-generation sequencing – single-copy gene.

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Ketersediaan

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Informasi Detail

Judul Seri

Biological Journal of the Linnean Society

No. Panggil

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Penerbit

: The Linnean Society of London., 2016

Deskripsi Fisik

Biological Journal of the Linnean Society, 2016, 117, 71–82. With 3 figures.

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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