e-journal

Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding

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Freshwater fauna are particularly sensitive to environmental change and disturbance. Management agencies frequently
use fish and amphibian biodiversity as indicators of ecosystem health and a way to prioritize and assess
management strategies. Traditional aquatic bioassessment that relies on capture of organisms via nets, traps and
electrofishing gear typically has low detection probabilities for rare species and can injure individuals of protected
species. Our objective was to determine whether environmental DNA (eDNA) sampling and metabarcoding analysis
can be used to accurately measure species diversity in aquatic assemblages with differing structures. We manipulated
the density and relative abundance of eight fish and one amphibian species in replicated 206-L mesocosms.
Environmental DNA was filtered from water samples, and six mitochondrial gene fragments were Illuminasequenced
to measure species diversity in each mesocosm. Metabarcoding detected all nine species in all treatment
replicates. Additionally, we found a modest, but positive relationship between species abundance and sequencing
read abundance. Our results illustrate the potential for eDNA sampling and metabarcoding approaches to improve
quantification of aquatic species diversity in natural environments and point the way towards using eDNA metabarcoding
as an index of macrofaunal species abundance.

Keywords: community ecology, environmental DNA, mesocosm, metabarcoding, species diversity
Received 23 March 2015; revision received 19 May 2015; accepted 26 May 2015

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Informasi Detail

Judul Seri

Molecular Ecology Resources

No. Panggil

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Penerbit

: John Wiley & Sons, Ltd.., 2016

Deskripsi Fisik

Molecular Ecology Resources (2016) 16, 29–41

Bahasa

English

ISBN/ISSN

doi: 10.1111/1755-09

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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