e-journal

The impact of library preparation protocols on the consistency of allele frequency estimates in Pool-Seq data

---

Sequencing pools of individuals (Pool-Seq) is a cost-effective method to determine genome-wide allele frequency
estimates. Given the importance of meta-analyses combining data sets, we determined the influence of different
genomic library preparation protocols on the consistency of allele frequency estimates. We found that typically no
more than 1% of the variation in allele frequency estimates could be attributed to differences in library preparation.
Also read length had only a minor effect on the consistency of allele frequency estimates. By far, the most pronounced
influence could be attributed to sequence coverage. Increasing the coverage from 30- to 50-fold improved
the consistency of allele frequency estimates by at least 27%. We conclude that Pool-Seq data can be easily combined
across different library preparation methods, but sufficient sequence coverage is key to reliable results.

Keywords: Drosophila, NGS libraries, Pool-Seq, population genetics—empirical

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Molecular Ecology Resources

No. Panggil

-

Penerbit

: John Wiley & Sons, Ltd.., 2016

Deskripsi Fisik

Molecular Ecology Resources (2016) 16, 118–122

Bahasa

English

ISBN/ISSN

doi: 10.1111/1755-09

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

BIOLOGI

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar