e-journal

Comparative transcriptome resources of two Dysosma species (Berberidaceae) and molecular evolution of the CYP719A gene in Podophylloideae

---

Dysosma species (Berberidaceae, Podophylloideae) are of great medicinal pharmacogenetic importance and used as
model systems to study the drivers and mechanisms of species diversification of temperate plants in East Asia.
Recently, we have sequenced the transcriptome of the low-elevation D. versipellis. In this study, we sequenced the
transcriptome of the high-elevation D. aurantiocaulis and used comparative genomic approaches to investigate the
transcriptome evolution of the two species. We retrieved 53 929 unigenes from D. aurantiocaulis by de novo transcriptome assemblies using the Illumina HiSeq 2000 platform. Comparing the transcriptomes of both species, we
identified 4593 orthologs. Estimation of Ka/Ks ratios for 3126 orthologs revealed that none had a Ka/Ks significantly
greater than 1, whereas 1273 (Ka/Ks < 0.5, P < 0.05) were inferred to be under purifying selection. A total of 51 primer pairs were successfully designed from 461 EST-SSRs contained in 4593 orthologs. Marker validation assay revealed
that 26 (51%) and 41 (80.4%) produced clear fragments with the expected sizes in all Podophylloideae species. Specifically,19 different sequences of CYP719A were identified from PCR-amplified genomic DNA of all 12 species of
Podophylloideae using primers designed from the assembled transcripts. The data further indicated that CYP719A
was likely subject to strong selective constraints maintaining only one copy per genome. In Dysosma, there was
relaxed purifying selection or more positive selection for high-elevation species. Overall, this study has generated a
wealth of molecular resources potentially useful for pharmacogenetic and evolutionary studies in Dysosma and
allied taxa.

Keywords: CYP719A, Dysosma, molecular evolution, podophyllotoxin, purifying selection, transcriptome

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Molecular Ecology Resources

No. Panggil

-

Penerbit

: John Wiley & Sons., 2016

Deskripsi Fisik

Molecular Ecology Resources (2016) 16, 228–241

Bahasa

English

ISBN/ISSN

doi: 10.1111/1755-09

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

BIOLOGI

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar