e-journal

Measuring telomere length and telomere dynamics in evolutionary biology and ecology

---

1. Telomeres play a fundamental role in the protection of chromosomal DNA and in the regulation of cellular
senescence. Recent work in human epidemiology and evolutionary ecology suggests adult telomere length (TL)
may reflect past physiological stress and predict subsequent morbidity and mortality, independent of
chronological age.
2. Several differentmethods have been developed to measure TL, each offering its own technical challenges. The
aim of this review is to provide an overview of the advantages and drawbacks of each method for researchers,
with a particular focus on issues that are likely to face ecologists and evolutionary biologists collecting samples in the fieldor inorganisms thatmay neverhave been studied in this context before.
3. We discuss the key issues to consider and wherever possible try to provide current consensus view regarding
best practice with regard to sample collection and storage, DNA extraction and storage, and the five main
methods currently available tomeasure TL.
4. Decisions regarding which tissues to sample, how to store them, how to extractDNA, and which TL measurement
method to use cannot be prescribed, and are dependent on the biological question addressed and the constraints
imposed by the study system. What is essential for future studies of telomere dynamics in evolution and
ecology is that researchers publish full details of theirmethods and the quality control thresholds they employ.

Key-words: DNA extraction, dot blot, fluorescent in situ hybridization, life history, quantitative
real-time PCR, senescence, single telomere length analysis, telomerase, telomere restriction fragment
analysis

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

Methods in Ecology and Evolution

No. Panggil

-

Penerbit

: British Ecological Society., 2014

Deskripsi Fisik

Methods in Ecology and Evolution 2014, 5, 299–310

Bahasa

English

ISBN/ISSN

doi: 10.1111/2041-21

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

BIOLOGI

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar