e-journal

simMSG: an experimental design tool for high-throughput genotyping of hybrids

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Hybridization between closely related species, whether naturally occurring or laboratory generated, is a useful tool
for mapping the genetic basis of the phenotypic traits that distinguish species. The development of next-generation
sequencing techniques has greatly improved our ability to assign ancestry to hybrid genomes. One such next-generation
sequencing technique, multiplexed shotgun genotyping (or MSG), can be a powerful tool for genotyping
hybrids. However, it is difficult a priori to predict the accuracy of MSG in natural hybrids because accuracy depends
on ancestry tract length and number of ancestry informative markers. Here, we present a simulator, ‘simMSG’, that
will allow researchers to design MSG experiments and show that in many cases MSG can accurately assign ancestry
to hundreds of thousands of sites in the genomes of natural hybrids. The simMSG tool can be used to design experiments
for diverse applications including QTL mapping, genotyping introgressed lines or admixture mapping.

Keywords: hybridization, multiplexed shotgun genotyping, natural hybrids, reduced representation genotyping

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Informasi Detail

Judul Seri

Molecular Ecology Resources

No. Panggil

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Penerbit

: John Wiley & Sons., 2016

Deskripsi Fisik

Molecular Ecology Resources (2016) 16, 183–192

Bahasa

English

ISBN/ISSN

doi: 10.1111/1755-09

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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