e-journal
Phylogeographic insights of the lowland species Cheirolophus sempervirens in the southwestern Iberian Peninsula
The southwestern Iberian Peninsula is an important biogeographic region, showing high biodiversity
levels and hosting several putative glacial refugia for European flora. Here, we study the genetic diversity and
structure of the Mediterranean, thermophilous plant Cheirolophus sempervirens (Asteraceae) across its whole
distribution range in SW Iberia, as a tool to disentangle some of the general biogeographic patterns shaping this
southern refugia hotspot. Null genetic diversity was observed in the cpDNA sequencing screening. Nonetheless,
AFLP data revealed high levels of among-population genetic differentiation correlated to their geographic location.
Our results suggest longer species persistence in southern Iberian refugia during glacial periods and subsequent
founder effects northwards due to colonizations in warmer stages (i.e., the southern richness to northern purity
pattern). Additionally, our phylogeographic analyses indicate the presence of two separate genetic lineages within
Ch. sempervirens, supporting the hypothesis of multiple minor refugia for SW Iberia in agreement with the refugia
within refugia model.
Key words: AFLP, genetic diversity, genetic structure, glacial refugia, Mediterranean, Pleistocene climatic oscillations, southern Europe.
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