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Museomics illuminate the history of an extinct,paleoendemic plant lineage (Hesperelaea, Oleaceae) known from an 1875 collection from Guadalupe Island,Mexico

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Museum collections are essential for understanding biodiversity and next-generation sequencing methods (NGS)
offer new opportunities to generate genomic data on specimens of extinct species for phylogenetic and other
studies. Hesperelaea is a monotypic Oleaceae genus that was collected only once, 140 years ago on Guadalupe
Island, Mexico. This lineage is almost certainly extinct, and has been considered an insular paleoendemic of
unknown relationship within subtribe Oleinae. Here, a genome skimming approach was attempted on the
H. palmeri specimen to generate genomic data in order to interpret the biogeographic history of Hesperelaea in a
phylogenetic framework. Despite highly degraded DNA, we obtained the complete plastome, the nuclear
ribosomal DNA cluster (nrDNA), and partial sequences of low-copy genes. Six plastid regions and nrDNA internal
transcribed spacers were used for phylogenetic estimations of subtribe Oleinae, including data from previous
studies. Bayesian and maximum likelihood phylogenies strongly place Hesperelaea within an American lineage
that includes Forestiera and Priogymnanthus. Molecular dating suggests an Early Miocene divergence between
Hesperelaea and its closest relatives. Our study thus confirms that Hesperelaea was a paleoendemic lineage that
likely predates Guadalupe Island, and provides a notable example of the high potential of NGS for analyzing
historical herbarium specimens and revolutionizing systematics.

ADDITIONAL KEYWORDS: California flora – chloroplast DNA – genome assembly – historic herbarium collections – nuclear ribosomal DNA – phylogenetics.

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Ketersediaan

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Informasi Detail

Judul Seri

Biological Journal of the Linnean Society

No. Panggil

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Penerbit

: The Linnean Society of London., 2016

Deskripsi Fisik

Biological Journal of the Linnean Society, 2016, 117, 44–57. With 2 figures

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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