e-journal

Using nuclear gene data for plant phylogenetics: Progress and prospects II. Next-gen approaches

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Single and low copy nuclear genes offer a larger number of, and more rapidly evolving, characters than
the chloroplast and nuclear ribosomal gene sequences that have dominated plant phylogenetic studies to date.
Until recently, only one or a few low copy nuclear gene markers were included in such studies. Now, the rapid
adoption of “next generation sequencing” (NGS) techniques offers simpler and cheaper access to hundreds of, and
not just tens of, coding and noncoding DNA regions. In this review, we describe the most commonly-used NGS
methods available for accessing nuclear genes and discuss many NGS case studies that have been published in the
last two to three years. These approaches include whole genome sequencing to target microsatellites,
transcriptome sequencing, Exon-Primed Intron-Crossing sequencing (EPIC), targeted enrichment (or sequence
capture), RAD sequencing (RAD-Seq, including genotyping-by-sequencing or GBS), and genome skimming. We also
discuss some of the challenges to, and posed by, the NGS approaches.

Key words: next generation sequencing, NGS, nuclear genes, phylogenetics, phylogenomics.

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Ketersediaan

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Informasi Detail

Judul Seri

J. Syst. Evol.

No. Panggil

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Penerbit

: Institute of Botany., 2015

Deskripsi Fisik

J. Syst. Evol. 53 (5): 371–379, 2015

Bahasa

English

ISBN/ISSN

doi: 10.1111/jse.121

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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