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New ultra-performance liquid chromatographytandem mass spectrometry method for the determination of irbesartan in human plasma

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With the objective of reducing analysis time and maintaining good efficiency, there has been substantial focus on high-speed chromatographic separations and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) is a preeminent analytical tool for rapid biomedical analysis. In this study a simple, rapid, sensitive, and
specific ultra-performance liquid chromatography-MS/MS method was developed and validated for quantification of the angiotensin II receptor antagonist, irbesartan (IRB), in human plasma. After a simple protein precipitation using methanol and acetonitrile, IRB and internal standard (IS) telmisartan were separated on Acquity UPLC BEH C18 column
(50 mm
2.1 mm, i.d. 1.7 mm, Waters, Milford, MA, USA) using a mobile phase consisted of acetonitrile: methanol: 10 mM ammonium acetate (70: 15: 15 v/v/v) with a flow rate of 0.4 mL/min and detected MS/MS in negative ion mode. The ion transitions recorded in multiple reaction monitoring mode were m/z 427.2/193.08 for IRB and m/z 513.2/469.3 for
IS. The assay exhibited a linear dynamic range of 2e500 ng/mL for IRB in human plasma with good correlation coefficient of (0.995) and with a lower limit of quantitation of 2 ng/mL. The intra- and interassay precisions were satisfactory; the relative standard deviations did not exceed 9.91%. The proposed UPLC-MS/MS method is simple, rapid, and highly sensitive, and hence it could be reliable for pharmacokinetic and toxicokinetic study in both animals and humans

Keywords:
high throughput analysis irbesartan pharmacokinetic tandem mass spectrometry toxicokinetic ultra-performance liquid
chromatography

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Ketersediaan

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Informasi Detail

Judul Seri

Journal Of Food and Drug Analysis

No. Panggil

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Penerbit

Taiwan : Elsevier Taiwan., 2014

Deskripsi Fisik

Journal Of Food and Drug Analysis 22 (2014) 569-576

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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