High performance liquid chromatography (HPLC)fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry

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High performance liquid chromatography (HPLC)fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry

Chi Wang [et al.] - Nama Orang;

Corn peptides (CPs) are reported to have many biological functions, such as facilitating alcohol metabolism, antioxidation, antitumor, antihypertension, and hepatoprotection. To develop a method for quality control, the high-performance liquid chromatography (HPLC) system was applied. Twenty-eight common peaks were found in all the CPs of corn samples from Enshi, China, based on which, a fingerprinting chromatogram was established for use in quality control in future research. Subsequently, the major chemical constituents of these common peaks were identified respectively using the HPLC-diodearray detection electrospray ionization tandem mass spectrometry (DAD-ESI-MS/MS) system,and 48 peptide fractions were determined ultimately. This was the first time for the majority of these peptides to be reported, and many of them contained amino acids of glutamine (Q), L and A, which might play an important role in the exhibition of the bioactivities of CPs. Many peptides had a similar primary structure to the peptides which had
been proven to be bioactive such as facilitating alcohol metabolism, scavenging free radicals,and inhibiting lipid peroxidation. This systematical analysis of the primary structure of CPs facilitated subsequent studies on the relationship between the structures and functions, and could accelerate holistic research on CPs.

Keywords:
corn peptides electrospray ionization mass spectrometry (ESI-MS) high-performance liquid chromatography (HPLC)
HPLC fingerprints primary structure

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Judul Seri: Journal Of Food and Drug Analysis
No. Panggil: -
Penerbit: Taiwan : Elsevier Taiwan., 2014
Deskripsi Fisik: Journal Of Food and Drug Analysis 22 (2014) 95-104
Bahasa: English
ISBN/ISSN: -
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: -
Subjek: Food And Health
Info Detail Spesifik: -
Pernyataan Tanggungjawab: Wati/Agus

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FULL TEXT:High performance liquid chromatography (HPLC)fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry

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