e-journal

Tetrodotoxin and paralytic shellfish poisons in gastropod species from Vietnam analyzed by highperformance liquid chromatography and liquid chromatographyetandem mass spectrometry

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Among marine toxins, tetrodotoxin (TTX) and paralytic shellfish poisons (PSPs) are known as notorious neurotoxins that induce serious food poisoning incidents in the Southeast Asia region. The aim of this study was to investigate whether TTX and PSP toxins are important issues of seafood safety. Paralytic toxicity was observed in mice exposed to 34 specimens from five species of gastropods using a PSP bioassay. Five species of gastropods,Natica vitellus, Natica tumidus, Oliva hirasei, Oliva lignaria, and Oliva annulata, were collected from the coastal seawaters in Nha Trang City, Vietnam, between August 2007 and October 2007. The average lethal potency of gastropod specimens was 90
40 (mean
standard deviation) mouse units (MU) for N. vitellus, 64
19 MU for N. tumidus, 42
28 MU for O. hirasei, 51
17 MU for O. lignaria, and 39
18 MU for O. annulata. All toxic extracts from the sample species were clarified using a C18 Sep-Pak solid-phase extraction column and a microcentrifuge filter prior to analysis. High-performance liquid chromatography coupled with fluorescence detection indicated that the toxins of the olive shell (O. hirasei, O. lignaria,and O. annulata) were mainly composed of saxitoxin (STX) (73e82%), gonyautoxin (GTX) 2, 3 (12e22%), and minor levels of TTX (5e6%). The toxins of N. vitellus and N. tumidus were mainly composed of STX (76e81%) and GTX 1, 4 (19e24%). Furthermore, liquid chromatographyetandem mass spectrometry analysis was used to verify the identity of the PSPs and TTX. Our evidence shows that these gastropods have novel toxin profiles

Keywords:
High-performance liquid chromatography coupled with fluorescence detection Liquid chromatographyetandem mass spectrometry Paralytic shellfish poison Tetrodotoxin

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Informasi Detail

Judul Seri

j o u rnal of food and drug a n a l y s i s

No. Panggil

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Penerbit

: Elsevier Taiwan., 2014

Deskripsi Fisik

j o u rnal of food and drug a n a l y s i s 2 2 ( 2 0 1 4 ) 1 7 8 e1 8 8

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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