e-journal

Development and validation of a simple reversedphase HPLC-UV method for determination of oleuropein in olive leaves

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A simple, precise, accurate, and selective method is developed and validated for the determination of oleuropein, which is the main phenolic compound in olive leaves. Separation was achieved on a reversed-phase C18 column (5 mm, 150
4.6 mm inner diameter) using a mobile phase consisting of acetonitrile/phosphate buffer pH 3.0 (20:80, v/v), at a flow rate of 1.0 mL/minute and UV detection at 280 nm. This method is validated according to the requirements for new methods, which include accuracy, precision, selectivity, robustness,limit of detection, limit of quantitation, linearity, and range. The current method demonstrates good linearity over the range of 3e1000 ppm of oleuropein, with r2 > 0.999. The recovery of oleuropein in olive leaves ranges from 97.7% to 101.1%. The method is selective, in
that oleuropein is well separated from other compounds of olive leaves with good resolution. The method is also precisedthe relative standard deviation of the peak areas of replicate injections of oleuropein standard solution is

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Ketersediaan

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Informasi Detail

Judul Seri

j ournal of food and drug anal ys i s

No. Panggil

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Penerbit

: Elsevier Taiwan., 2014

Deskripsi Fisik

j ournal of food and drug anal ys i s 2 2 ( 2 0 1 4 ) 2 8 5 e2 8 9

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

-

Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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