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Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on compositioneactivity relationship approach

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Camellia chrysantha (Hu) Tuyama (CCT), an ornamental plant possessing antioxidant activity, has been infused as tea and drank for its health benefits. The antioxidant components in CCT, however, had not been clearly characterized. To quickly identify the antioxidant constituents of CCT, a compositioneactivity relationship strategy based on
ultra high-pressure liquid chromatography coupled with linear ion trap hybrid orbitrap mass spectrometry and orthogonal partial least-squares method has been applied. As a result, 16 variables were found to make significant contributions to the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Six of them were identified as catechin (1),
epicatechin (5), vitexin (8), isovitexin (10), quercetin-7-O-b-D-glucopyranoside (12) and kaempferol (16). The strength of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was found to be 12 > 1 > 5 > 16 > 8 > 10 by validation test. Meanwhile, a liquid chromatography-electrospray ionization-mass spectrometry method was established for
quantitative determination of six marker compounds in CCT samples from different preparations. The validation of the method, including linearity, sensitivity (limitation of detection and limitation of quantification), repeatability, precision, stability, and recoveries,was carried out and demonstrated to meet the requirements of quantitative
analysis. This is the first report on the comprehensive characterization and determination of chemical constituents in CCT by ultra high-pressure liquid chromatography coupled with linear ion trap hybrid orbitrap mass spectrometry. The results indicate that the compositioneactivity relationship approach may be a useful method for the discovery of active constituents in natural plants and the quality control of medicinal herbs.

Keywords:
antioxidant components Camellia chrysantha (Hu) Tuyama compositioneactivity relationship ultra high-pressure liquid chromatography (UHPLC) linear ion trap hybrid orbitrap mass spectrometry

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Informasi Detail

Judul Seri

j o u rnal of food and drug a n a l y s i s

No. Panggil

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Penerbit

: Elsevier Taiwan., 2015

Deskripsi Fisik

j o u rnal of food and drug a n a l y s i s 2 3 ( 2 0 1 5 ) 4 0 e4 8

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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