Analysis of major antioxidants from extracts of Myrmecodia pendans by UV/visible spectrophotometer, liquid chromatography/tandem mass spectrometry, and high-performance liquid chromatography/UV techniques

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Analysis of major antioxidants from extracts of Myrmecodia pendans by UV/visible spectrophotometer, liquid chromatography/tandem mass spectrometry, and high-performance liquid chromatography/UV techniques

Adam Mekonnen Engida [et al.] - Nama Orang;

In the present work, heat reflux extraction with ethanol/water (80:20; v/v) as the solvent was used to extract antioxidants fromMyrmecodia pendans. Thecrude extract (CE) wasfractionated using hexane and ethyl acetate. Ethyl acetate fraction (EAF) and aqueous fraction were collected. Antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl-radical radicalandferric reducing power of the CE, EAF, and aqueous fraction were evaluated. EAF showed comparable
antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl-radical radical and ferric reducing power to those of the CE. UV/visible, liquid chromatography/electrospray ionization/tandem mass spectrometry, and high-performance liquid chromatography were employed for identifying the major antioxidant compounds in the EAF. Three major phenolic compounds
(rosmarinic acid, procyanidin B1,andpolymerof procyanidin B1)were identified. Thefirsttwo compounds were confirmed and quantified by high-performance liquid chromatography using authentic standards, but confirmation of the thirdcompoundwashampered by a lack of commercial standard. Concentrations of rosmarinic acid and procyanidin B1 in the EAF were found to be 20.688± 1.573 mg/g drysampleand 3.236±0.280 mg/g drysample, respectively. All these three compounds are reported for the first time in sarang semut.

Keywords:antioxidant high-performance liquid chromatography/tandem mass spectrometry Myrmecodia pendans reducing power

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Judul Seri: j ournal of food and drug anal ys i s
No. Panggil: -
Penerbit: : Elsevier Taiwan., 2015
Deskripsi Fisik: j ournal of food and drug anal ys i s 2 3 ( 2 0 1 5 ) 3 0 3 e3 0 9
Bahasa: English
ISBN/ISSN: -
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: -
Subjek: Food And Health
Info Detail Spesifik: -
Pernyataan Tanggungjawab: Wati/Agus

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FULL TEXT:Analysis of major antioxidants from extracts of Myrmecodia pendans by UV/visible spectrophotometer, liquid chromatography/tandem mass spectrometry, and high-performance liquid chromatography/UV techniques

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