Development and validation of a high-performance thin layer chromatography method for the determination of cholesterol concentration

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Development and validation of a high-performance thin layer chromatography method for the determination of cholesterol concentration

Jinu John [et al.] - Nama Orang;

An accurate, sensitive, precise, reliable, and quick method for the determination of cholesterol content by high-performance thin layer chromatography is developed. In this method, aluminum-backed precoated silica gel 60 F254 plates were used as the stationary phase and the samples were sprayed with the help of CAMAG sample applicator Linomat 5.
The chromatogram was developed with the mobile phase consisting of chloroform:methanol (9.5:0.5, v/v). The samples were detected using CAMAG Scanner 4 and evaluated using the method developed on winCATS software. Densitometric analysis of
cholesterol was performed in absorbance mode at 200 nm. In this solvent system,cholesterol gave a compact spot with an Rf value of 0.63 ± 0.03. The linear regression analysis of data for the calibration curve showed good linearity over a concentration range of 2e7 mg/spot with a regression value of 0.99933 and standard deviation of 1.44%. The limit
of detection and limit of quantification were found to be 100 ng/spot and 500 ng/spot,respectively. Using the developed method, the concentration of cholesterol in the saponified and unsaponified egg yolk sample was determined. This method was found to be reproducible and can even be used for samples containing complex matrices.

Keywords:
cholesterol densitometric analysis egg yolk high-performance thin layer chromatography method

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Informasi Detail

Judul Seri: j ournal of food and drug anal ys i s
No. Panggil: -
Penerbit: : Elsevier Taiwan., 2015
Deskripsi Fisik: j ournal of food and drug anal ys i s 2 3 ( 2 0 1 5 ) 2 1 9 e2 2 4
Bahasa: English
ISBN/ISSN: -
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: -
Subjek: Food And Health
Info Detail Spesifik: -
Pernyataan Tanggungjawab: Wati/Agus

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FULL TEXT:Development and validation of a high-performance thin layer chromatography method for the determination of cholesterol concentration

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