e-journal

Clove (Syzygium aromaticum) ingredients affect lymphocyte subtypes expansion and cytokine profile responses: An in vitro evaluation

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Clove (Syzygium aromaticum) has been used in folk medicine in many disorders. The present work aimed to investigate effects of clove essential oil as eugenol and water soluble ingredients on mouse splenocytes. Clove extracts were harvested and in different concentrations (0.001e1000 mg/mL) were affected to splenocytes and also phytohemagglutinin
(PHA ¼ 5 mg/mL) and lipopolysaccharide (LPS ¼ 10 mg/mL) activated splenocytes; then splenocytes proliferation assayed using theMTT ([3-(4, 5-dimethylthiazole-2-yl) -2, 5-diphenyl tetrazolium bromide]) method were done. On the culture supernatant interferon (IFN)-g, interleukin (IL)-4, IL-10, and transforming growth factor (TGF)-b cytokines were measured. Clove ingredients (100 mg/mL and 1000 mg/mL) reduced PHA stimulated splenocytes proliferation and enhanced LPS stimulated cells expansion. Treated splenocytes showed suppression of IFN-g release and induction of IL-4, IL-10, and TGF-b secretion (in the range of 0.1e1000 mg/mL). The results of this study suggest clove extracts could suppress the T cell cellular immunity and enhance humoral immune responses. In clove affection cytokine pattern shifted
toward modulatory and Th2 responses and accelerator of humoral immunity cytokines

Keywords:
B cells Clove extract Cytokine In vitro Splenocytes T cells

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Ketersediaan

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Informasi Detail

Judul Seri

j o u rnal of food and drug a n a l y s i s

No. Panggil

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Penerbit

: Elsevier Taiwan., 2014

Deskripsi Fisik

j o u rnal of food and drug a n a l y s i s 2 2 ( 2 0 1 4 ) 4 4 8 e4 5 4

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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