e-journal

Finding of polysaccharide-peptide complexes in Cordyceps militaris and evaluation of its acetylcholinesterase inhibition activity

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Acetylcholinesterase (AChE) inhibition enhances learning and cognitive ability for treatment of Alzheimer's disease. Polysaccharideepeptide complexes were identified in Cordyceps militaris (CPSPs) and characterized for their AChE inhibitory properties. Three polymers (CPSP-F1,-F2, and -F3) were extracted and separated by ultrasound-assisted extraction and diethylaminoethanol (DEAE)eSepharose CL-6B column chromatography. Polysaccharideepeptide complexes were identified by DEAEeSepharose CL-6B column chromatography and highperformance gel-filtration chromatography, Fourier transform infrared spectra, amino sugar composition analysis, and b-elimination reaction to identify olysaccharideepeptide bond categories. Separation of CPSP can increase AChE inhibitory activity from the crude polysaccharide of C. militaris. CPSP-F1 and CPSP-F2 exhibited half maximal inhibitory concentrations of 32.2 ± 0.2 mg/mL and 5.3 ± 0.0 mg/mL. Thus, we identified polysaccharideepeptide complexes from C. militaris and suggest CPSP has great potential in AChE inhibition bioassay.

Keywords:
acetylcholinesterase Cordyceps militaris Fourier transform infrared spectra high-performance gel-filtration
chromatography polysaccharideepeptide complexes

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Informasi Detail

Judul Seri

j ournal of food and drug anal ys i s

No. Panggil

-

Penerbit

: Elsevier Taiwan., 2015

Deskripsi Fisik

j ournal of food and drug anal ys i s 2 3 ( 2 0 1 5 ) 6 3 e7 0

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

-

Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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