e-journal

Effect of storage timeonmetaboliteprofileandalphaglucosidase inhibitory activity of Cosmos caudatus leaves e GCMS basedmetabolomics approach

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Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “UlamRaja”. It is a localMalaysian herb traditionally used as a food andmedicinal herb to treat severalmaladies. Its bioactive or nutritional constituents consist of awide range ofmetabolites,including glucosinolates, phenolics, amino acids, organic acids, and sugars. However, many of these metabolites are not stable and easily degraded or modified during storage. In order to investigate the metabolomics changes occurring during post-harvest storage, C. caudatus samples were subjected to seven different storage times (0 hours, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, and 12 hours) at roomtemperature.As themodel experiment, themetabolites identified by gas chromatography-mass spectrometry (GC-MS) were correlated with a-glucosidase inhibitory activity analyzed with multivariate data analysis (MVDA) to find out the
variation among samples and metabolites contributing to the activity. Orthogonal partial least squares (OPLS) analysis was applied to investigate the metabolomics changes. A profound chemical alteration, both in primary and secondary metabolites, was observed. The a-tocopherol,catechin, cyclohexen-1-carboxylic acid, benzoic acid, myo-inositol, stigmasterol, and lycopenecompoundswerefoundtobethediscriminatingmetabolitesatearlystorage;however, sugarssuchassucrose, a-D-galactopyranose, and turanosewere detected,whichwas attributed to the discriminatingmetabolites for late storage. The result shows that theMVDA method is a promising technique to identify biomarker compounds relative to storage at different times.

Keywords:
Cosmos caudatus GC-MS MVDA OPLS storage

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Informasi Detail

Judul Seri

j ournal of food and drug anal ys i s

No. Panggil

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Penerbit

: Elsevier Taiwan., 2015

Deskripsi Fisik

j ournal of food and drug anal ys i s 2 3 ( 2 0 1 5 ) 4 3 3 e4 4 1

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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