e-journal

Effect of casein glycomacropeptide on subunit p65 of nucleartranscription factor-B in lipopolysaccharide-stimulatedhuman colorectal tumor HT-29 cells

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Objective: The effect of casein glycomacropeptide (CGMP) on the expression of NF-B subunit p65 in human colorectal cancer HT-29 cellsinduced by lipopolysaccharide (LPS) was investigated to explore the therapeutic efficacy of CGMP for human colorectal cancer.Methods: HT-29 cells cultured in 96-well plates were stimulated with LPS for 30 min at the concentrations of 0, 0.001, 0.01, 0.1, 1 and 10 g/mL.After stimulation, the expression of p65 in HT-29 cells was evaluated by immunofluorescence method. Similarly, HT-29 cells were incubated with0, 0.001, 0.01, 0.1, 1, 10 and 100 g/mL CGMP for 24 h at the optimal LPS concentration. After treatment with CGMP under the incubation withoptimal LPS concentration for 30 min, the protein expression of p65 was analyzed by Western blotting. The optimum culture time was evaluatedby incubating HT-29 cells with the optimal CGMP concentration for 6, 12, 24, 48 and 72 h.Results: NF-B-p65 revealed that the highest protein expression was achieved with 1 g/mL LPS treatment. Meanwhile, CGMP could inhibitthe protein expression of NF-B-p65 in LPS-stimulated HT-29 cells, and the optimal inhibitory effect was observed at a CGMP concentration of0.01 g/mL with 48 h incubation.Conclusion: CGMP can regulate NF-B signaling pathway through inhibiting the expression of its subunit p65, which is beneficial for the furtherimprovement of human colorectal cancer treatment.

Keywords: Casein glycomacropeptide (CGMP); Human colorectal tumor cell HT-29; Lipopolysaccharide; p65

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Informasi Detail

Judul Seri

Food Science and Human Wellness

No. Panggil

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Penerbit

: Elsevier B.V.., 2014

Deskripsi Fisik

Food Science and Human Wellness 3 (2014) 51–55

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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