e-journal

Detection of Salmonella and several common Salmonella serotypes in foodby loop-mediated isothermal amplification method

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Salmonella Choleraesuis, S. Enteritidis and S. Typhimurium are the main pathogens that contaminate animal products and cause human Salmonellafood poisoning. To establish the loop-mediated isothermal amplification (LAMP) method for the rapid detection of Salmonella and 3 commonSalmonella serotypes, inner and outer primer sets targeting Salmonella invE gene and 3 serotype-specific genes fliC, lygD and STM4495 weredesigned. The LAMP reaction conditions were optimized. The specificity of LAMP primers was identified by testing 10 different bacterialstrains including S. Choleraesuis, S. Enteritidis and S. Typhimurium. Take S. Choleraesuis as example, the detection limit of LAMP assay was1.33 × 101CFU/mL for bacteria culture and 2.0 × 101CFU/mL for simulated pork sample. The results show that LAMP is a rapid, sensitive andspecific method for Salmonella detection and can be used for the rapid detection of Salmonella in food.

Keywords: Loop-mediated isothermal amplification; Detection; Salmonella; Salmonella Choleraesuis; Salmonella Enteritidis; Salmonella Typhimurium

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Ketersediaan

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Informasi Detail

Judul Seri

Food Science and Human Wellness

No. Panggil

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Penerbit

: Elsevier B.V.., 2015

Deskripsi Fisik

Food Science and Human Wellness 4 (2015) 75–79

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Food And Health

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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