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Histological evaluation of low-intensity pulsed ultrasound on osteochondritis dissecans of the humeral capitellum

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Background: The clinical use of low-intensity pulsed ultrasound (LIPUS) was recently evaluated in cases of osteochondritis dissecans of the humeral capitellum (elbow OCD). However, the mechanism underlying the effect of LIPUS in elbow OCD is not well understood. The aim of this study was to histopathologically evaluate the effect of LIPUS irradiation on elbow OCD.
Methods: Fifteen patients with elbow OCD were enrolled in this study. All patients were juvenile baseball players (average age, 13.1 years).LIPUS was performed under the same conditions as the fracture treatment for an average length of 15.1 days in the preoperative period in seven patients (LIPUS group). Cylindrical tissue specimens obtained at the time of surgery were stained with hematoxylin and eosin and alcian blue,and were also immunostained to detect type 1 collagen (Col-1), osteopontin (OPN), and Runx2. The state of the cartilage and subchondral bone
and expression levels of Col-1, OPN, and Runx2 were evaluated with a semiquantitative grading system by a blinded pathologist. Histological and immunohistological findings in both groups were compared using Fisher’s exact test.
Results: Both groups showed reparative tissue and cartilaginous metaplasia at the separation level near the subchondral bone; Col-1 was expressed in the reparative tissue. Furthermore, OPN and Runx2 were expressed in the interstitial cells near the separation level. The cartilage and subchondral bone findings in histological evaluations did not differ significantly between the LIPUS and control groups. The distribution of OPN expression
levels in the two groups was as follows: Grade 0dLIPUS group, zero patients, and control group, five patients; Grade 1dLIPUS group and control group, two patients each; Grade 2dLIPUS group, five patients and control group, one patient; Grade 3dLIPUS group, one patient and control group, zero patients. OPN expression was significantly higher in the LIPUS group than in the control group ( p ¼ 0.04). Conclusion: LIPUS stimulation increased the expression levels of OPN in elbow OCD.

Keywords: immunohistochemistry; low-intensity pulsed ultrasound; osteochondritis dissecans of the humeral capitellum; osteopontin

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Informasi Detail

Judul Seri

Asia-Pacific Journal of Sports Medicine, Arthroscopy, Rehabilitation and Technology

No. Panggil

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Penerbit

: Asia Pacific Knee., 2015

Deskripsi Fisik

Asia-Pacific Journal of Sports Medicine, Arthroscopy, Rehabilitation and Technology 2 (2015) 56e62

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

SOSIAL

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

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