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Determination of fat-soluble vitamins in vegetable oils through microwave-assisted high-performance liquid chromatography

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In this manuscript, a study of the effect of microwave radiation on the high-performance
liquid chromatography separation of tocopherols and vitamin K1 was conducted. The novelty
of the application was the use of a relatively low polarity mobile phase in which the
dielectric heating effect was minimized to evaluate the nonthermal effect of the microwave
radiation over the separation process. Results obtained show that microwave-assisted highperformance
liquid chromatography had a shorter analysis time from 31.5 to 13.3 min when
the lowest microwave power was used. Moreover, narrower peaks were obtained; hence the
separation was more efficient maintaining or even increasing the resolution between the
peaks. This result confirms that the increase in mobile phase temperature is not the only
variable for improving the separation process but also other nonthermal processes must
intervene. Fluorescence detection demonstrated better signal-to-noise compared to photodiode
arrayed detection mainly due to the independent effect of microwave pulses on the
baseline noise, but photodiode array detection was finally chosen as it allowed a simultaneous
detection of nonfluorescent compounds. Finally, a determination of the content of the
vitamin E homologs was carried out in different vegetable oils. Results were coherent with
those found in the literature.

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Informasi Detail

Judul Seri

SEPARATION SCIENCE

No. Panggil

-

Penerbit

: JOURNAL OF SEPARATION SCIENCE., 2015

Deskripsi Fisik

J. Sep. Sci. 2015, 38, 1073–1081

Bahasa

English

ISBN/ISSN

1615-9306/DOI 10.100

Klasifikasi

-

Tipe Isi

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Tipe Media

-

Tipe Pembawa

-

Edisi

38, 1073–1081

Subjek

SEPARATION SCIENCE

Info Detail Spesifik

-

Pernyataan Tanggungjawab

deliza

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