e-journal

Visualization of O-GlcNAc Glycosylation Stoichiometry and Dynamics Using Resolvable Poly(ethylene glycol) Mass Tags

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O-linked N-acetylglucosamine (O-GlcNAc) glycosylation is a dynamic protein posttranslational modification with roles in processes such as transcription, cell cycle regulation, and metabolism. Detailed mechanistic studies of O-GlcNAc have been hindered by a lack of methods for measuring O-GlcNAc stoichiometries and the interplay of glycosylation with other posttranslational modifications. We recently developed a method for labeling O-GlcNAc-modified proteins with resolvable poly(ethylene glycol) mass tags. This mass-tagging approach enables the direct measurement of glycosylation stoichiometries and the visualization of distinct O-GlcNAc-modified subpopulations. Here, we describe procedures for labeling O-GlcNAc glycoproteins in cell lysates with mass tags.

Keywords: O-linked N-acetylglucosamine  glycosylation  posttranslational modifications  chemoenzymatic labeling  poly(ethylene glycol)  protein subpopulations

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Ketersediaan

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Informasi Detail

Judul Seri

Current Protocols in Chemical Biology

No. Panggil

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Penerbit

London : John Wiley & Sons, Ltd.., 2013

Deskripsi Fisik

Curr. Protoc. Chem. Biol. 5:281-302

Bahasa

English

ISBN/ISSN

DOI: 10.1002/9780470

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

5: December 2013

Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

agus

Versi lain/terkait

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