Cinnamon extract inhibits angiogenesis in zebrafish and human endothelial cells by suppressing VEGFR1, VEGFR2, and PKC-mediated MAP kinase

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Cinnamon extract inhibits angiogenesis in zebrafish and human endothelial cells by suppressing VEGFR1, VEGFR2, and PKC-mediated MAP kinase

Rishipal R. Bansode [et al.] - Nama Orang;

Angiogenesis is a process of new blood vessel generation and under pathological conditions, lead to tumor development, progression, and metastasis. Many bioactive components have been studied for its antiangiogenic properties as a preventive strategy against tumor development. This study is focused on the effects of cinnamon extract in modulating the pathway involved in angiogenesis.
Human umbilical vein endothelial cells (HUVEC) were treated with cinnamon extract at a concentration of 25 lg/mL for 1, 3, or 6 h followed by treatment with phorbol ester (TPA) at a concentration of 10 nmol/L to induce mitogenactivated protein kinase (MAPK) expression. Results show that cinnamon extract inhibited TPA-induced phosphorylation of MAPK and AKT in a dosedependent
manner. Gene expression results in HUVEC showed that cinnamon extract treatment inhibited TPA induction of protein kinase C, PKCa and PKCg messenger RNA (mRNA) expression in a dose-dependent manner along with suppression of vascular endothelial growth factor receptor 1 (VEGFR1/ Flt1) and vascular endothelial growth factor receptor 2 (VEGFR2/KDR/Flk1) mRNA expression. Cinnamon extract was administered to zebrafish embryos during gastrulation at 6–8 h post fertilization (hpf). The embryos were observed for changes in morphology, toxicity, and blood vessel development. The interseg mental vessels in the zebrafish embryos were attenuated and underdeveloped at an effective cinnamon extract dose of 250 lg/mL compared with the DMSOtreated control. Exposure to cinnamon extract for 36 h resulted in gross morphological deformities. The results suggest the effect of cinnamon extract on angiogenesis is mediated by PKC-dependent phosphorylation of MAPK.

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Judul Seri: Food Science & Nutrition
No. Panggil: -
Penerbit: : Food Science & Nutrition published by Wiley Periodicals, Inc., 2013
Deskripsi Fisik: Food Science & Nutrition 2013; 1(1): 74–82
Bahasa: English
ISBN/ISSN: doi: 10.1002/fsn3.13
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: 2013; 1(1)
Subjek: Food Science & Nutrition
Info Detail Spesifik: -
Pernyataan Tanggungjawab: deliza

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FULLTEXT: Cinnamon extract inhibits angiogenesis in zebrafish and human endothelial cells by suppressing VEGFR1, VEGFR2, and PKC-mediated MAP kinase

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