e-journal

Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

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The maize mutant gene Vestigial glume 1 (Vg1) has been fine-mapped to a narrow region by ap-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of 574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of themaize genome. Fosmid stability assays indicated that clones were stable during propagation in the
fosmid system. Using Vg1 candidate gene-specific primers, a positive clone was successfully identified. This discovery will pave the way for identifying the function of Vg1 in maize development.

Keywords:
Vestigial glume 1 Fosmid Library construction Library screening

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Informasi Detail

Judul Seri

T H E C R O P J O U R N A L

No. Panggil

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Penerbit

: Elsevier., 2016

Deskripsi Fisik

T H E C R O P J O U R N A L 4 ( 2 0 1 6 ) 5 5 – 6 0

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

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