e-journal
RL3(t), Responsible for Leaf Shape Formation, Delimited to a 46-kb DNA Fragment in Rice
Two mutants with rolled leaves, temporally designated as rl3(t)-1 and rl3(t)-2, were served for exploring
the mechanism underlying the rolled leaf characteristic. Except for having typical rolled leaves, the plant heights
and panicle lengths of rl3(t)-1 and rl3(t)-2 significantly decreased, and the seed-setting rate also decreased
when compared with wild type 93-11. Cytological analysis suggested that the rolled leaf phenotype might be caused
by the changes of number and size of bulliform cells. Genetic analysis indicated rl3(t)-1 is allelic to rl3(t)-2, and
controlled by a recessive gene. Gene mapping result indicated that RL3(t) gene resided in a 46-kb long region
governed by the sequence tag site markers S3-39 and S3-36 on rice chromosome 3. The result provides an
important clue for further cloning the RL3(t) and understanding the mechanism of rice leaf development.
Key words: gene mapping; leaf shape formation; mutant; rice; rolled leaf gene.
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