A Simple Plate Method for the Screening and Detection of Fucoidanases

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A Simple Plate Method for the Screening and Detection of Fucoidanases

Artem S. Silchenko [et al.] - Nama Orang;

A novel method was developed for the screening and detection of fucoidanase activity in bacterial colonies using fucoidan–agarose plates and staining with hexadecyltrimethylammonium bromide (Cetavlon). Colonies with fucoidanase activity were indicated by a transparent halo. The medium containing undegraded fucoidan showed as a milk-colored background, but areas with degraded fucoidan under the colonies were visualized as a transparent halo.

Keywords:
Bacteria Fucoidanase Fucoidan Plate assay Fucoidanase activity assay Carbohydrate polyacrylamide gel electrophoresis

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Judul Seri: Achievements in the Life Sciences
No. Panggil: -
Penerbit: : Elsevier., 2015
Deskripsi Fisik: Achievements in the Life Sciences 9 (2015) 104–106
Bahasa: English
ISBN/ISSN: -
Klasifikasi: NONE
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: 9 ( 2015 )
Subjek: KIMIA
Info Detail Spesifik: -
Pernyataan Tanggungjawab: Wati/Agus

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FULL TEXT:A Simple Plate Method for the Screening and Detection of Fucoidanases

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