Nucleic Acid and Protein Profile of Bacteriophages that Infect Pseudomonas syringae pv. glycinea, Bacterial Blight on Soybean

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Nucleic Acid and Protein Profile of Bacteriophages that Infect Pseudomonas syringae pv. glycinea, Bacterial Blight on Soybean

Hardian Susilo Addy [et al.] - Nama Orang;

Several management strategies of Bacterial blight disease caused by Pseudomonas syringae pv. glycinea has been developing to minimize yield loss including the use of bacteriophage as phage therapy. However, some consideration must be point on its interaction with bacterial host and influence of bacterial host virulence. Therefor, information of characteristic of prospective bacteriophage must be determined prior to use as biological control agent. This research aimed to obtain some information about general characteristics of bacteriophage that infects P. syringae pv. glycinea such as the ability in propagation in bacterial host,type of nucleic acid, and total protein profile. The results showed that about four bacteriophages have been isolated from soybean soil in Sukorambi and Mangli area named φSK2a, φSK2b, φSK2c (from Sukorambi), and φMGX1 (from Mangli). All phages had different ability in propagation in bacterial host, P. syringae pv. glycinea strain H3 about 1010-1012 PFU/mL. Nucleic acid analysis using digestion enzymes (DNase, RNase, and Restriction endonuclease) showed that all bacteriophage isolates had double stranded deoxyribonucleic acid (dsDNA). Moreover, protein profile of bacteriophage through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that all phages had similar pattern of the protein suggested the same type of bacteriophage. However, morphological characteristic is still needed to accurate determination of bacteriophage taxonomy.

Keywords: P. syringae pv. glycinea; bacterial blight; soybean; bacteriophage

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Judul Seri: Agriculture and Agricultural Science Procedia
No. Panggil: -
Penerbit: : Graduate School of Economics, Waseda University., 2016
Deskripsi Fisik: Agriculture and Agricultural Science Procedia 9 ( 2016 ) 475 – 481
Bahasa: English
ISBN/ISSN: doi: 10.1016/j.aaspr
Klasifikasi: NONE
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: 9 ( 2016 )
Subjek: KIMIA
Info Detail Spesifik: -
Pernyataan Tanggungjawab: Wati/Agus

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FULL TEXT:Nucleic Acid and Protein Profile of Bacteriophages that Infect Pseudomonas syringae pv. glycinea, Bacterial Blight on Soybean

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