e-journal

Identification of EST–SSRs and molecular diversity analysis in Mentha piperita

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EST sequences of Mentha piperita available in the public domain (NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of 1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent (72.3%) with the AG/CT (43.8%) repeat motif followed by AT/AT (16.2%). Primers were successfully designed for 68 SSR-containing sequences (62.0%). The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33 (61%) were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of 2.33 alleles/SSR, and the polymorphic information content (PIC) value varied between 0.13
and 0.51 (average 0.25). All the amplified SSRs showed transferability among four different species of Mentha, with a highest in Mentha arvensis (87.0%) and minimum in Mentha citrata (37.0%). The newly developed SSRs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.

Keywords:
EST–SSRs Genetic diversity Mentha piperita Polymorphic information content Transferability

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Informasi Detail

Judul Seri

T H E C R O P J O U R N A L

No. Panggil

-

Penerbit

: Elvesier., 2015

Deskripsi Fisik

T H E C R O P J O U R N A L 3 ( 2 0 1 5 ) 3 3 5 – 3 4 2

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

-

Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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