e-journal
Genomewide association study of Aegilops tauschii traits under seedling-stage cadmium stress
Aegilops tauschii Ais a wild relative of commonwheat (Triticum aestivum) and acts as an important resource of elite genes including genes for resistance to biotic and abiotic stresses. To improve the cadmium(Cd) tolerance ofwheat varieties using A. tauschii resources, we investigated the genetic variation of biomass-based Cd tolerance in 235 A. tauschii accessions treated with 0 (control) and 100 μmol L−1 CdCl2 (as Cd stress). Simultaneously, we performed a genomewide association study (GWAS) using a single-nucleotide polymorphism chip containing 7185 markers. Six
markerswere found to be significantly associatedwith Cd tolerance by a general linear model and a mixed linear model. These markers were close to several candidate/flanking genes associated with Cd tolerance according to results in public databases, including pdil5-1, Acc-1, DME-5A,TaAP2-D, TaAP2-B, Vrn-B1, and FtsH-like protein gene. The A. tauschii accessions were classified as high, moderate, and low Cd-tolerant according to a secondary index, the synthetic index (SI), in proportions of 9%, 57%, and 34%, respectively. By the average SI, accessions from Afghanistan,Turkey, Azerbaijan, and Iran showed relatively high Cd tolerance.
Keywords:Aegilops tauschii GWAS Cadmium tolerance Biomass
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